FastQCFastQC Report
Wed 14 May 2025
MODEK_ctrl_r1_100k.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMODEK_ctrl_r1_100k.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100000
Total Bases5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA3031330.313000000000002RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1155311.552999999999999RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA58565.856RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG24812.481RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG21532.153RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA18421.8419999999999999RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC16441.644RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC14751.4749999999999999RNA PCR Primer, Index 1 (100% over 27bp)
TAGGTAGTTTCATGTTGTTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA12231.2229999999999999RNA PCR Primer, Index 1 (100% over 28bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG10211.0210000000000001RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA9430.943RNA PCR Primer, Index 1 (100% over 28bp)
GAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG9150.915RNA PCR Primer, Index 1 (100% over 29bp)
CGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA8180.818RNA PCR Primer, Index 1 (100% over 32bp)
CTAGACTGAGGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA7640.764RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG7600.76RNA PCR Primer, Index 1 (100% over 29bp)
GAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGAACTCC6690.6689999999999999RNA PCR Primer, Index 1 (100% over 27bp)
AGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAG6050.605RNA PCR Primer, Index 1 (100% over 29bp)
AAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGAACTCCA5520.5519999999999999RNA PCR Primer, Index 1 (100% over 28bp)
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACATGGAATTCTCGGGTGC5300.53No Hit
CCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5170.517RNA PCR Primer, Index 1 (100% over 31bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG5140.514RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA5060.506No Hit
TGAGTGTGTGTGTGTGAGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG4620.462RNA PCR Primer, Index 1 (100% over 29bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG4360.436Illumina Small RNA Adapter 2 (100% over 21bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA3750.375No Hit
TGAGGTAGTAGGTTGTGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT3650.365RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGGAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3500.35000000000000003RNA PCR Primer, Index 1 (100% over 29bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCC3190.319No Hit
GAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACATGGAATTCTCGGGTG3110.311No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC3020.302RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGGAGGTTGTATAGTTGTGGAATTCTCGGGTGCCAAGGAACTCC2910.291RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC2580.258RNA PCR Primer, Index 1 (100% over 26bp)
GAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG2380.23800000000000002RNA PCR Primer, Index 1 (100% over 29bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2190.219RNA PCR Primer, Index 1 (100% over 31bp)
CTAGACTGAGGCTCCTTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT2070.207RNA PCR Primer, Index 1 (100% over 30bp)
CGCAATGAAGGTGAAGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1810.181RNA PCR Primer, Index 1 (100% over 30bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC1720.172RNA PCR Primer, Index 1 (100% over 24bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1720.172RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1600.16No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1570.157RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1550.155RNA PCR Primer, Index 1 (100% over 30bp)
TAGGTAGTTTCATGTTGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG1540.154RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC1530.153RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG1510.151Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC1420.14200000000000002RNA PCR Primer, Index 1 (100% over 27bp)
GACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1410.14100000000000001RNA PCR Primer, Index 1 (100% over 30bp)
GCGGTCGGCGTCCCCCAACTTCTTAGAGGGACATGGAATTCTCGGGTGCC1410.14100000000000001No Hit
CCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1410.14100000000000001RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1390.13899999999999998RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1360.136No Hit
CCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1330.133RNA PCR Primer, Index 1 (100% over 30bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT1310.131RNA PCR Primer, Index 1 (100% over 25bp)
TAGGTAGTTTCATGTTGTTGGGATGGAATTCTCGGGTGCCAAGGAACTCC1310.131RNA PCR Primer, Index 1 (100% over 27bp)
CTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGTGCCAAGGA1280.128RNA PCR Primer, Index 1 (100% over 22bp)
TGAGTGTGTGTGTGTGAGTGTGTTGGAATTCTCGGGTGCCAAGGAACTCC1250.125RNA PCR Primer, Index 1 (100% over 27bp)
GAGCCGTTCGTCTTGGGAAATGGAATTCTCGGGTGCCAAGGAACTCCAGT1200.12RNA PCR Primer, Index 1 (100% over 30bp)
CACTAGATTGTGAGCTGCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA1130.11299999999999999RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA1100.11RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG1090.109No Hit
GGGGCTGGAGAGATGGCTTAGTGGAATTCTCGGGTGCCAAGGAACTCCAG1010.101RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)