FastQCFastQC Report
Sat 30 Oct 2021
MODEK_100k.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMODEK_100k.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA3032630.325999999999997RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1183211.831999999999999RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA59215.921RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG25372.537RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG22372.237RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA19321.932RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC17231.7229999999999999RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC14231.423RNA PCR Primer, Index 1 (100% over 27bp)
TAGGTAGTTTCATGTTGTTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA12811.281RNA PCR Primer, Index 1 (100% over 28bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG10261.026RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA9950.9950000000000001RNA PCR Primer, Index 1 (100% over 28bp)
GAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG8970.897RNA PCR Primer, Index 1 (100% over 29bp)
CGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA7760.776RNA PCR Primer, Index 1 (100% over 32bp)
CTAGACTGAGGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA7450.745RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG7110.711RNA PCR Primer, Index 1 (100% over 29bp)
GAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGAACTCC6830.683RNA PCR Primer, Index 1 (100% over 27bp)
AGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAG6020.602RNA PCR Primer, Index 1 (100% over 29bp)
AAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGAACTCCA5400.54RNA PCR Primer, Index 1 (100% over 28bp)
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACATGGAATTCTCGGGTGC5300.53No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG5300.53RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA5040.504No Hit
CCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5010.501RNA PCR Primer, Index 1 (100% over 31bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG4760.47600000000000003Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGGAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3980.398RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT3620.362RNA PCR Primer, Index 1 (100% over 30bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA3170.317No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCC3100.31No Hit
TGAGGTAGGAGGTTGTATAGTTGTGGAATTCTCGGGTGCCAAGGAACTCC2970.297RNA PCR Primer, Index 1 (100% over 27bp)
GAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACATGGAATTCTCGGGTG2950.295No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC2920.292RNA PCR Primer, Index 1 (100% over 26bp)
GAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG2650.265RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC2500.25RNA PCR Primer, Index 1 (100% over 26bp)
TGAGTGTGTGTGTGTGAGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2470.247RNA PCR Primer, Index 1 (100% over 29bp)
CGCAATGAAGGTGAAGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1890.189RNA PCR Primer, Index 1 (100% over 30bp)
CTAGACTGAGGCTCCTTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1850.185RNA PCR Primer, Index 1 (100% over 30bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC1670.167RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG1660.166Illumina Small RNA Adapter 2 (100% over 21bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1610.161RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1600.16RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1590.159No Hit
GAGCCGTTCGTCTTGGGAAATGGAATTCTCGGGTGCCAAGGAACTCCAGT1590.159RNA PCR Primer, Index 1 (100% over 30bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC1580.158RNA PCR Primer, Index 1 (100% over 26bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC1470.147RNA PCR Primer, Index 1 (100% over 24bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1430.14300000000000002RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1390.13899999999999998No Hit
TAGGTAGTTTCATGTTGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG1380.13799999999999998RNA PCR Primer, Index 1 (100% over 29bp)
CCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1380.13799999999999998RNA PCR Primer, Index 1 (100% over 32bp)
GCGGTCGGCGTCCCCCAACTTCTTAGAGGGACATGGAATTCTCGGGTGCC1370.13699999999999998No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT1330.133RNA PCR Primer, Index 1 (100% over 25bp)
TAGGTAGTTTCATGTTGTTGGGATGGAATTCTCGGGTGCCAAGGAACTCC1320.132RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG1270.127No Hit
CTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGTGCCAAGGA1250.125RNA PCR Primer, Index 1 (100% over 22bp)
GACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1220.122RNA PCR Primer, Index 1 (100% over 30bp)
CCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1200.12RNA PCR Primer, Index 1 (100% over 30bp)
CTTAGAACTGGTGCGGACCAGGGGAATCCTGGAATTCTCGGGTGCCAAGG1120.11199999999999999Illumina Small RNA Adapter 2 (100% over 21bp)
CACTAGATTGTGAGCTGCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA1090.109RNA PCR Primer, Index 1 (100% over 28bp)
GGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1060.106RNA PCR Primer, Index 1 (100% over 31bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1040.104RNA PCR Primer, Index 1 (100% over 31bp)
ATGGTTCCTTTGGTCGCTCGCTCCTTGGAATTCTCGGGTGCCAAGGAACT1010.101RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)